Journal: Pharmaceuticals
Article Title: IL-13Rα2 Is Involved in Resistance to Doxorubicin and Survival of Osteosarcoma Patients
doi: 10.3390/ph17111526
Figure Lengend Snippet: Effect of IL-13Rα2 on migration and invasion activity of osteosarcoma cells: ( A ) Migration assay was conducted by seeding 1 × 10 5 cells into the upper chamber of each well. ( B ) Invasion assays were performed by seeding 2 × 10 5 cells in each well of the Matrigel-coated upper chamber. Serum-free media was used in the upper chamber for both migration and invasion assays, while the lower chamber contained media with 10% serum to serve as a chemoattractant. The migrated or invaded cells were stained with Diff Quick staining solution and counted in five separate ×100 microscopic fields per well. Original magnification, ×400. *** p < 0.001; EV, empty vector for wild-type IL-13Rα2; IL-13Rα2-OE, vector for wild-type IL-13Rα2; shControl, control vector for shRNA; shIL-13Rα2, vector for shRNA for IL-13Rα2.
Article Snippet: Cells that migrated or invaded to the lower side of the membrane were stained with Diff Quick stain solution (#468.037.1A, Diff-Quick, Dade Behring Inc., Newark, DE, USA).
Techniques: Migration, Activity Assay, Staining, Diff-Quik, Plasmid Preparation, Control, shRNA